Coding
MazE

Part:BBa_K4060000

Designed by: YU CHUAN LEE   Group: iGEM21_NYCU-Taipei   (2021-09-30)


MazE gene

Function of MazE MazE is an antitoxin,which binds to MazF and stops MazF from killing the cell.

Usage and Biology We use this part to apply in our kill switch system and stop MazF from cleaving the mRNA ACA site.

Experience and Result We use modeling to analyze the interaction between MazE and MazF. The result shows that the mRNA of MazF is less vulnerable to MazF. That is to say MazF is not good at recognizing the mRNA MazE. Hence, MazE can be expressed fluently under the pressure of MazF. We suggest that’s why MazE can serve as the antitoxin gene toward the MazF toxin gene.

Pre A1 is an intermediate construct for kill switch system.

Characterization and Improving Function

Team: NYCU_Taipei 2021

Author: Yi-Rong Chen


Agarose electrophoresis result

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4
    Illegal XbaI site found at 20
    Illegal SpeI site found at 274
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4
    Illegal SpeI site found at 274
    Illegal NotI site found at 11
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4
    Illegal BamHI site found at 71
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4
    Illegal XbaI site found at 20
    Illegal SpeI site found at 274
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4
    Illegal XbaI site found at 20
    Illegal SpeI site found at 274
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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